Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems [full article]
Evan Murray, Jae Hun Cho, Daniel Goodwin, Taeyun Ku, Justin Swaney, Sung-Yon Kim, Heejin Choi, Jeong-Yoon Park, Austin Hubbert, Meg McCue, Young-Gyun Park, Sara Vassallo, Naveed Bakh, Matthew Frosch,, Van J. Wedeen, H. Sebastian Seung, and Kwanghun Chung. Simple, scalable proteomic imaging for high-dimensional profiling of intact systems, Cell, Dec 3:163(6): 1500-14. doi: 10.1016/j.cell.2015.11.025. PubMed PMID: 26638076.
The SWITCH tissue processing framework consists of three key methods:
- Scalable and uniform tissue preservation
- Easy and rapid tissue clearing
- Virtually unlimited rounds of antibody labeling, stripping, and relabeling.
With these methods, you can perform many labeling rounds on the same tissue. Better yet, this is scalable to large volumes.